Coding

Part:BBa_K093015:Design

Designed by: John Heil   Group: iGEM08_Waterloo   (2009-04-20)


phi C31 integrase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1665
    Illegal SapI.rc site found at 1356
    Illegal SapI.rc site found at 1414
    Illegal SapI.rc site found at 1519


Design Notes

Removed EcoRI site via site directed mutagenesis, replaced non standard start codon, and replaced non standard stop codon.


Source

PCR amplified from pHS62

Thorpe, H. M. & Smith, M. C. (1998). In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc Natl Acad Sci U S A 95, 5505-5510.

References